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PUC19 Vector Sequence Primer

This post categorized under Vector and posted on June 8th, 2019.

Plasmid pUC19 from Dr. Joachim Messings lab is published in Gene. 1983 Dec26(1)101-6. This plasmid is available through Addgene.Close This website uses cookies to ensure you get the best experience. By continuing to use this site you agree to the use of cookies.Although a very large number of host organisms and molecular cloning vectors are in use the great majority of molecular cloning experiments begin with a laboratory strain of the bacterium E. coli (Escherichia coli) and a plasmid cloning vector.

The pENTRD-TOPO Cloning Kits utilize a highly efficient 5-minute cloning strategy (TOPO Cloning) to directionally clone a blunt-end PCR product into a vector for entry into the Gateway System or the MultiSite Gateway System.We highly recommend using our web tool NEBuilder to design PCR primers with overlapping sequences between the adjacent DNA fragments and for their graphicembly into a cloning vector.

NEBuilder HiFi DNA graphicembly Cloning Kit was developed to improve the efficiency and accuracy of DNA graphicembly. This method allows for seamless graphicembly of multiple DNA fragments regardless of fragment graphicgth or end compatibility.

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